635 PB 334 HISTOCHEMICAL LOCALIZATION OF LIPOXYGENASE, PEROXIDASE AND POLYPHENOL OXIDASE IN POTATO TUBER TISSUE
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چکیده
منابع مشابه
Purification and Characterization of Polyphenol Oxidase, Peroxidase and Lipoxygenase from Freshly Cut Lettuce (L. sativa)
Enzymatic reactions taking place in minimally processed vegetables are considered as a major problem, because they adversely affect sensorial and nutritional quality. Polyphenol oxidase (PPO), peroxidase (POD) and lipoxygenase (LOX) from lettuce were purified on a column packed with positively charged diethylaminoethyl (DEAE) cellulose by applying pH gradient elution from pH=4.0 to 9.0. The mai...
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Thin sections of wound-healing potato tuber tissue were stained with rabbit antibody prepared against a suberization-associated anionic peroxidase and then stained with a goat anti-rabbit antibody-fluorescein conjugate. When these sections were examined with an epiilluminating fluorescence microscope, bright green fluorescent linear deposits were observed on the inner side of cell walls in the ...
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A crude enzyme extract from Dioscorea esculenta var. fasiculata tissue subjected to ion exchange chromatography on DEAE-Sephadex A-50 column. This procedure resolved the extract into two main protein peaks one of which eluted through the column relatively unbound while the other protein peak which remained bound to the column was eluted with 1.0 M NaCl. Both protein peaks contained polyphenol o...
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Plant lipoxygenases (LOXs) are a functionally diverse class of dioxygenases implicated in physiological processes such as growth, senescence, and stress-related responses. LOXs incorporate oxygen into their fatty acid substrates and produce hydroperoxide fatty acids that are precursors of jasmonic acid and related compounds. Here, we report the involvement of the tuber-associated LOXs, designat...
متن کاملPotato tuber tissue respiration & ventilation.
The purpose of this work is to compare gas movement through potato tuber tissue with the respiratory rate of such tissue and to assess possible oxygen deficiency as a factor in the limitation of respiration within the tissue. Argon 37 was used as a substitute for oxygen in most of the gas transfer experiments because it is easier to measure, its solubilities and diffusion coefficients are very ...
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ژورنال
عنوان ژورنال: HortScience
سال: 1994
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.29.5.523e